Journal: ACS Applied Materials & Interfaces

Article Title: Polymer Casting and Water Immersion-Based Large-Area Graphene Transfer for Flexible Electronics Fabrication

doi: 10.1021/acsami.5c23601

Figure Lengend Snippet: Live/Dead cell assay analysis at 7 days on (A) Control hAD-MSCs and (B) hAD-MSCs on patterned graphene/PLA films. DNA damage analysis using a comet assay at 7 days on (C) Control hAD-MSCs and (D) hAD-MSCs on patterned graphene/PLA films. (E) Cell viability analysis of hAD-MSCs on patterned graphene/PLA films and Control at 7 days. (F) Apoptosis analysis on hAD-MSCs on patterned graphene/PLA films at 7 days. Cell cycle analysis on (G) Control hAD-MSCs and (H) hAD-MSCs on patterned graphene/PLA films at 7 days. (I) ICC analysis of stem cell markers CD29 and CD44 for hAD-MSCs growing on patterned graphene/PLA films and Petri dishes (control) at 7 days.

Article Snippet: Human adipose-derived mesenchymal stem cells (hAD-MSCs) obtained from ATCC (PCS-500–011) were cultured in mesenchymal stem cell basal medium (ATCC, PCS-500–030) containing 2.1% of MSC supplement (ATCC, PCS-999–038), 1.2% of l -alanyl- l -glutamine (ATCC, PCS-999–034), and 0.1% of penicillin-streptomycin amphotericin B solution (ATCC, PCS-999–002) in a sterile 75 cm 2 cell culture flask, kept in an incubator at 5% CO 2 and a temperature of 37 °C.

Techniques: Control, Single Cell Gel Electrophoresis, Cell Cycle Assay

Journal: Psycho-Oncology

Article Title: The Psychological Impact of Screen‐Detected Cancer: A Systematic Review

doi: 10.1002/pon.70358

Figure Lengend Snippet: Anxiety (using HADS‐A or STAI) and Depression (using HADS‐D or CES‐D) from studies that reported means by detection route over time. Where cut‐offs for anxiety were reported, these ranged from > 8 to > 11 (for HADS‐A); > 44 (for the STAI); > 8 to > 14 (for HADS‐D) and > 16 (for CES‐D); Data beyond 24 months from diagnosis are not presented here but can be seen in supporting Information.

Article Snippet: Ellman (1995), Breast [ ] , HADS‐D: 7 items, scores calculated by simple addition, scores of 7/8 indicates borderline and 10/11 indicates definite depression. Total score range 0–21. , Not stated (long‐term survivors) (SnD: n = 205; non‐SND: n = 126) , Not presented for SnD alone , — , No significant difference (data not shown) [Insufficient data to calculate].

Techniques: Biomarker Discovery

Journal: Psycho-Oncology

Article Title: The Psychological Impact of Screen‐Detected Cancer: A Systematic Review

doi: 10.1002/pon.70358

Figure Lengend Snippet: Depression among participants with screen‐detected cancer reported over time using the HADS‐D or CES‐D. *Depression scores were reported for sub‐groups of those with screen‐detected cancer (e.g., by treatment) so an overall mean was calculated for presentation. Where cut‐offs for depression were reported, these ranged from > 8 to > 14 (for HADS‐D) and > 16 (for CES‐D); Data beyond 24 months from diagnosis are not presented here but can be seen in supporting Information.

Article Snippet: Ellman (1995), Breast [ ] , HADS‐D: 7 items, scores calculated by simple addition, scores of 7/8 indicates borderline and 10/11 indicates definite depression. Total score range 0–21. , Not stated (long‐term survivors) (SnD: n = 205; non‐SND: n = 126) , Not presented for SnD alone , — , No significant difference (data not shown) [Insufficient data to calculate].

Techniques: Biomarker Discovery

Journal: PLOS Neglected Tropical Diseases

Article Title: Endothelial cells protect Schistosoma mansoni from hydrogen peroxide-induced death

doi: 10.1371/journal.pntd.0013923

Figure Lengend Snippet: The percentage of nonviable worms was assessed following exposure to H₂O₂ (400 µM), with or without the addition of catalase (CAT), DDS-NOH (50 µM), and/or HUVECs. Catalase reduced the mortality induced by H₂O₂, an effect that was reversed in the presence of DDS-NOH, suggesting catalase inhibition. Interestingly, co-culture with HUVECs preserved worm viability even in the presence of DDS-NOH, indicating that HUVECs may provide additional protective mechanisms beyond catalase activity. Bars represent the percentage of nonviable worms in each experimental condition; horizontal lines indicate statistically significant differences (p < 0.05, Fisher’s exact test).

Article Snippet: Dapsone hydroxylamine (Santa Cruz Biotechnology, TX, USA) was tested in three different in vitro experimental models.

Techniques: Inhibition, Co-Culture Assay, Activity Assay

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Schematic illustrations of electrically potentiated MSCs (epMSCs) and their improved angiogenic potentials.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques:

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Electrical stimulation (ES) of hAD‐MSCs. (A) Schematic illustrations of in vitro experimental procedures and ES parameters. A constant voltage of 0.3 V with low frequency (LF, 1 Hz) and high frequency (HF, 100 Hz) was used for ES of hAD‐MSCs. (B) LIVE/DEAD staining images of the unstimulated MSCs (control) or MSCs stimulated with LF or HF. Green and red cells represent live and dead cells, respectively. Scale bars = 200 µm. (C) Cell viability of hAD‐MSCs ( n = 4). Cell viability was expressed as the percentage of live cells among the total (live and dead) cells. (D) Relative metabolic activity of unstimulated or electrically stimulated MSCs. Relative metabolic activity of each group was demonstrated as absorbance at 450 nm normalized by that of the unstimulated control ( n = 4).

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: In Vitro, Staining, Control, Activity Assay

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Expression of typical angiogenic markers in epMSCs. (A) Relative mRNA expression of VEGF‐A, HGF, bFGF, and IGF‐1 genes. Expression of each gene was normalized to that of GAPDH ( n = 4). (B) Quantification of VEGF‐A and HGF produced from MSCs and epMSCs ( n = 4). (C) Fold change in the mean pixel density of each growth factor produced by MSCs and epMSCs ( n = 4). CMs from MSCs and epMSCs were analyzed using a human growth factor antibody array to detect growth factor production in MSCs. Gene expression was normalized to GAPDH using the 2 −ΔΔCt method. An asterisk (*) denotes a statistically significant difference ( p < 0.05).

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: Expressing, Produced, Ab Array, Gene Expression

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Quantitative RNA sequencing analysis. (A) Volcano plot of differentially expressed genes (DEGs). The red and blue dots indicate the upregulated and downregulated genes in the epMSCs compared with those in the MSCs, respectively. (B) Hierarchical clustering analysis of DEGs between the MSCs and epSCs with a color scale indicating upregulation (red) and downregulation (blue). ( n = 3 per group). (C) Ten major gene ontology (GO) analysis of the genes of which expressions were significantly influenced by ES. (D) Gene expression profile associated with GO terms related to angiogenesis. Genes depicted in blue and red indicate upregulation and downregulation of angiogenesis, respectively.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: RNA Sequencing, Gene Expression

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Proteomics analysis of MSCs and epMSCs. (A) Venn diagram illustrating the distribution of proteins identified the CM of MSCs and epMSCs. (B) Hierarchical clustering analysis of the differentially expressed proteins between MSC CM and epMSC CM with a color scale indicating downregulation (blue) and upregulation (red). (C) GO analysis highlighting the top 10 biological processes significantly altered by ES with. (D) Functional enrichment analysis of differentially expressed proteins with the top 10 significantly enriched GO terms related to signaling pathways.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: Functional Assay, Protein-Protein interactions